temperature dependence of the ultrasonic parameters of bovine muscle: effects of muscle anisotropy
Dwyer K., Allen P. and Buckin, V. A.
Progress in Colloid and Polymer Science, 115, pp. 282-286 (2000)
Ultrasonic velocity and attenuation in muscle were measured as a function of temperature using a highresolution ultrasonic resonator technique. The effect of anisotropy on these ultrasonic parameters over the temperature ramp was also investigated. High resolution of our measurements enabled us to obtain the derivative of ultrasonic velocity as a function of temperature. The ultrasonic parameters in both muscles, with fibres of parallel and perpendicular orientation to the direction of propagation of ultrasonic waves, underwent profound changes with temperature. Muscles with fibres of parallel orientation showed several marked peaks in the velocity differential plots in the temperature interval between 280 and 315 K. The perpendicular samples showed significantly less temperature dependence. Attenuation increased in the parallel sample between 290 and 295 K and decreased between 300 and 310 K, however, these changes were absent in the sample with fibres of perpendicular orientation. Some of these transitions are well correlated with known phase transitions in muscle lipids and proteins. Overall, our results demonstrate that the resonator technique can be used to determine the structural changes in muscles with temperature.