the mastery of sound – high resolution ultrasonic spectroscopy for analysis of chemical reactions
Vitaly Buckin, Evgeny Kudryashov, Breda O'Driscoll
Lab News, February, pp. 9&25 (2002)
Ultrasonic spectroscopy is a non-destructive analytical technique based on the measurements of parameters of low energy ultrasonic waves. It is known through its successful applications in medicine and number of fields of material analysis. However, limited resolution of the measurements and large sample volume required prevented a wide spread of this technique in research and analytical laboratories in the past. Modern technologies employed in the new HR-US 101 high-resolution spectrometer from Ultrasonic Scientific Ltd. overcome these limitations, thus making commercial ultrasonic instruments available for a wide spectrum of analytical tasks. This instrument requires small sample volume (1 ml typically, down to 0.03 ml custom made) and performs analysis in various regimes with a record resolution. It was used at the Department of Chemistry University College Dublin in analysis of enzymatic reactions, conformational transitions in polymers, biopolymer-ligand binding and antigen-antibody interactions, aggregation in suspensions and emulsions, formation of particle and polymer gels, micellisation, adsorption on particle surfaces, composition analysis and others. Some of these applications are discussed below.